Coding

Part:BBa_K1179061:Design

Designed by: Kyle Lathem   Group: iGEM13_MIT   (2013-09-15)


eYFP-4x503ts


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 881
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 881
    Illegal SpeI site found at 857
    Illegal PstI site found at 238
    Illegal PstI site found at 765
    Illegal PstI site found at 788
    Illegal PstI site found at 811
    Illegal PstI site found at 834
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The miRNA503 doesn't completely repress the eYFP, some background expression is still visible.

Source

This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has been modified to fluoresce yellow and also synthesized with four miRNA503 target sites.

References